DETECTION OF ANTIBODIES AGAINST HUMAN PAPILLOMAVIRUS (HPV) TYPE-16 VIRIONS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING RECOMBINANT KPV-16-L1CAPSIDS PRODUCED BY RECOMBINANT BACULOVIRUS

Authors
LECANN P TOUZE A ENOGAT N LEBOULLEUX D MOUGIN C LEGRAND MC CALVET C AFOUTOU JM COURSAGET P
Citation
P. Lecann et al., DETECTION OF ANTIBODIES AGAINST HUMAN PAPILLOMAVIRUS (HPV) TYPE-16 VIRIONS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING RECOMBINANT KPV-16-L1CAPSIDS PRODUCED BY RECOMBINANT BACULOVIRUS, Journal of clinical microbiology, 33(5), 1995, pp. 1380-1382
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
5
Year of publication
1995
Pages
1380 - 1382
Database
ISI
SICI code
0095-1137(1995)33:5<1380:DOAAHP>2.0.ZU;2-I
Abstract
The L1 major capsid protein of human papillomavirus type 16 (HPV-16) w as expressed in Sf-21 insect cells with a recombinant baculovirus. Vir us-like particles obtained were purified and used to develop an enzyme -linked immunosorbent assay for detection of anti-HPV-16 antibodies in sera from 76 women with evidence of genital HPV infection and 79 cont rols. HPV-16-infected individuals developed antibodies directed at HPV -16 virions since reactivity against recombinant HPV-16L1 capsids was observed in 50% of them compared with only 6% in the general adult pop ulation. However, some cross-reactivities with sera from women infecte d with others HPV types were observed.