USE OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM AS A GENETIC-MARKER FOR TYPING MYCOBACTERIUM-AVIUM STRAINS

Authors
ROIZ MP PALENQUE E GUERRERO C GARCIA MJ
Citation
Mp. Roiz et al., USE OF RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM AS A GENETIC-MARKER FOR TYPING MYCOBACTERIUM-AVIUM STRAINS, Journal of clinical microbiology, 33(5), 1995, pp. 1389-1391
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
5
Year of publication
1995
Pages
1389 - 1391
Database
ISI
SICI code
0095-1137(1995)33:5<1389:UORAAG>2.0.ZU;2-9
Abstract
Restriction fragment length polymorphism (RFLP) was used to study 75 c linical isolates identified as Mycobacterium avium. Two repetitive ins ertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from pat ients with AIDS exhibited marked polymorphism with both probes.