DEVELOPMENT AND MAPPING OF 10 PORCINE MICROSATELLITE MARKERS

Thirty (TG)(n) microsatellite clones were isolated from a pig genomic library, sequenced, and tested for their suitability to detect polymor phism on a panel of animals by means of the polymerase chain reaction. Ten of these clones were developed into suitable markers and subseque ntly segregation of these markers was determined in the five PiGMaP re ference pedigrees. A linkage analysis was performed on these 10 micros atellites together with 365 other loci that have been typed on these r eference families. Eight of the microsatellites have been mapped to ei ght different linkage groups that have been previously assigned to dif ferent chromosomes (chromosomes 1, 6, 7, 9, 14, 15, 17 and 18). Of the remaining two markers, one is X-linked and the other shows no linkage . The number of alleles detected by these microsatellites, in the refe rence pedigrees, varied from six to sixteen and the heterozygosity var ied from 42 to 85% in the 26 unrelated founder animals of these refere nce pedigrees.