S. Shahabuddin, QUANTITATIVE DIFFERENCES IN CD8(+) LYMPHOCYTES, CD4 CD8 RATIO, NK CELLS, AND HLA-DR(+)-ACTIVATED T-CELLS OF RACIALLY DIFFERENT MALE POPULATIONS/, Clinical immunology and immunopathology, 75(2), 1995, pp. 168-170
In an attempt to establish the reference ranges for lymphocyte subsets
the distribution of lymphocyte-population-bearing surface markers suc
h as CD3 (T cells), CD19 (B cells), CD4 (T helper/inducer cells), CD8
(T suppressor/cytotoxic cells), HLA-DR on CD3(+) cells (activated T ce
lls), and CD16 and/or CD56 on CD3(-) cells (NK cells) has been studied
among normal male Saudi blood donors. Anticoagulated peripheral blood
was stained with monoclonal antibodies and the lymphocytes were analy
zed by flow cytometry for the expression of the above markers. Referen
ce ranges were as follows: CD3(+) (61 to 87%), CD19(+) (5 to 27%), CD4
(+) (31 to 55%), CD8(+) (24 to 56%), CD4(+)/CD8(+) ratio (0.5 to 1.8),
CD3(+) HLA-DR(+) (4 to 29%), and CD3(-) CD16(+)/CD56(+) (4 to 29%). F
urthermore, the absolute cell numbers for each lymphocyte subset are r
eported here for the first time for the Saudi male population. The mos
t significant finding of the present study is the presence of a higher
percentage and number of CD8(+) T cells (P < 0.01) and a decreased CD
4/CD8 ratio (P < 0.02) compared with the Caucasian controls. In additi
on, the Saudi male population has a significantly lower percentage and
number of activated T cells (P < 0.05 and < 0.01, respectively) and a
lower number of NK cells (P < 0.001). (C) 1995 Academic Press, Inc.