OPTIMIZED SEPARATION OF PESTICIDE ENANTIOMERS BY CHIRAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A computer-assisted method is described for optimization of multi-comp onent, mobile phase selection for separating enantiomers of four pesti cides in normal-phase HPLC. The method is based on the triangle, solve nt-selection concept using a statistical scanning method. The optimiza tion of the separation over the experimental region is based on a spec ial polynomial estimation from seven experimental runs, and resolution (R(S)) is used as the selection criterion. Excellent agreement was ob tained between predicted and experimental data.