HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF METHYL ETHYLKETONE IN URINE AS ITS 3-METHYL-2-BENZOTHIAZOLINONE HYDRAZONE DERIVATIVE

Authors
GORI G MENEGHETTI P STURARO A PARVOLI G DORETTI L BARTOLUCCI GB
Citation
G. Gori et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF METHYL ETHYLKETONE IN URINE AS ITS 3-METHYL-2-BENZOTHIAZOLINONE HYDRAZONE DERIVATIVE, Chromatographia, 40(5-6), 1995, pp. 336-340
Citations number
14
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
40
Issue
5-6
Year of publication
1995
Pages
336 - 340
Database
ISI
SICI code
0009-5893(1995)40:5-6<336:HLDOME>2.0.ZU;2-#
Abstract
A HPLC method for the determination of methyl ethyl ketone (MEK) in ur ine after derivatization with 3-methyl-2-benzothiazolinone hydrazone i s proposed. The calibration curve for the ketone was linear, ranging b etween 0.23-10 mg/L, with a detection limit of 0.025 mg/L. The results were compared to those obtained by GC-MS, coupled to the headspace te chnique. MEK derivatization and the derivative purification processes were verified with respect to the main variables such as reaction temp erature, reagent concentration, probable interferences and enrichment phase. The method is simple and reliable and shows a good sensitivity.