IDENTIFICATION AND DOSAGE OF URACIL AND 5 -HALOGENOURACIL IN SOLUTIONBY UV-SPECTROMETRY AND HPLC

Authors
ALISKANDARANI M VERNUS E COMEL C JOSE J PETITRAMEL M
Citation
M. Aliskandarani et al., IDENTIFICATION AND DOSAGE OF URACIL AND 5 -HALOGENOURACIL IN SOLUTIONBY UV-SPECTROMETRY AND HPLC, Analusis, 23(1), 1995, pp. 17-22
Citations number
12
Categorie Soggetti
Chemistry Analytical
Journal title
Analusis → ACNP
ISSN journal
03654877
Volume
23
Issue
1
Year of publication
1995
Pages
17 - 22
Database
ISI
SICI code
0365-4877(1995)23:1<17:IADOUA>2.0.ZU;2-G
Abstract
Successive deprotonations of uracil and 5-halogenouracils are displaye d in a wide pH range (6 < pH < 16). The conditional stability constant s and the molar absorbance of each protonated form have been determine d by simultaneous and spectrometric UV measurements. Besides, high per formance liquid chromatography (HPLC) allowed investigation of uracil chlorination by sodium hypochlorite solution in determined conditions. Advantages and disadvantages of both techniques are discussed and a c omparison made in terms of species detection.