MESOGLYCAN AND SULODEXIDE ACT AS STABILIZERS AND PROTECTORS OF FIBROBLAST GROWTH-FACTORS (FGFS)

Citation
M. Tardieu et al., MESOGLYCAN AND SULODEXIDE ACT AS STABILIZERS AND PROTECTORS OF FIBROBLAST GROWTH-FACTORS (FGFS), Growth factors, 11(4), 1994, pp. 291-300
Citations number
41
Categorie Soggetti
Biology
Journal title
ISSN journal
08977194
Volume
11
Issue
4
Year of publication
1994
Pages
291 - 300
Database
ISI
SICI code
0897-7194(1994)11:4<291:MASAAS>2.0.ZU;2-0
Abstract
Heparin and heparan sulfate proteoglycans (HSPGs) stabilize FGFs which belong to heparin-binding growth factors (HBGFs) on active conformati on. They also strongly potentiate their mitogenic activity on many cel l types, and protect them against thermal denaturation and enzymatic d egradation. In the present work we have tested two heparin-like substa nces named mesoglycan and sulodexide obtained from bovine intestinal m ucosal extracts. These products are used as heparin, in various of the rapeutic fields such as atherosclerosis or antithrombotic therapy. The compositions of mesoglycan and sulodexide are partially known and inc lude chondroitin, dermatan and heparan sulfate. We have shown that mes oglycan and sulodexide potentiated the mitogenic activity of FGF1 and FGF2. The magnitude of this effect was identical with that of heparin used as a control substance but at double concentration. Mesoglycan an d sulodexide also exerted stabilizing and protective effects on FGFs f or heat denaturation and enzymatic degradation. The suppression of the protective properties after heparinase treatment of mesoglycan and su lodexide indirectly demonstrated the presence of heparan sulfate which was shown to represent about 60% of the commercial products.