Mt. Gore et al., MYOSIN HEAVY-CHAIN GENE-EXPRESSION IN BOVINE FETUSES AND NEONATES REPRESENTING GENOTYPES WITH CONTRASTING PATTERNS OF GROWTH, Proceedings of the Society for Experimental Biology and Medicine, 209(1), 1995, pp. 86-91
Fetal and neonatal myosin heavy chain (MHC) gene expression was examin
ed in bovine genotypes that differed in their postnatal growth pattern
and mature size. Pregnancies were established that would be expected
to produce early-, intermediate-, or late-maturing postnatal growth. F
etal skeletal and cardiac muscles were collected at 100 and 200 days o
f gestation and at 30 days of age. Muscle tissue was analyzed for rela
tive levels of MHC RNA and protein. Longissimus muscle MHC RNA/mu g RN
A was greater at the 100-day time point for the intermediate maturity
type (P < 0.05), which differed from the 200-day time point where the
early maturity type had the greater RNA level (P < 0.05). Triceps musc
le MHC RNA/mu g RNA weights differed due to genotype at 200 days gesta
tion but did not differ at 100 days gestation or at 30 days postnatal.
Ventricular muscle MHC RNA did not differ due to genotype at any of t
he three developmental stages. Differences due to maturity type in MHC
protein/mg DNA were observed at 30 days of age, but no differences du
e to maturity type were observed at the prenatal time points. These re
sults indicate that bovine fetal skeletal muscle MHC RNA production ca
n be influenced by genotype and that genotype may be an important fact
or for future studies examining the role of external influences on fet
al muscle growth.