ANTIPLATETLET ACTIVATING FACTOR (PAF) ANTIBODY INHIBITS CFW MOUSE PREIMPLANTATION EMBRYO DEVELOPMENT

Objective: Our purpose was to investigate the effect of anti-PAF antib odies on CFW mouse embryo development in vitro. Design: We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage. Results: Mouse embryos cultured with anti-PAF (1:5 d ilution; 61%) significantly decreased embryo development compared To c ontrols (MEM alone; 93%), whereas embryos cultured in anti-mouse IgG-s upplemented MEM (1:10 dilution; 93%) had no effect. Conclusions: The r esults provide additional evidence that PAF is produced and secreted b y cleavage-stage embryos and is required during the preimplantation pe riod.