DECREASE OF 12-HYDROXYEICOSATETRAENOIC ACID PRODUCTION IN MOUSE LUNGSFOLLOWING DIETARY OLEIC ANILIDE CONSUMPTION - IMPLICATIONS FOR THE TOXIC OIL SYNDROME
Sh. Yoshida et al., DECREASE OF 12-HYDROXYEICOSATETRAENOIC ACID PRODUCTION IN MOUSE LUNGSFOLLOWING DIETARY OLEIC ANILIDE CONSUMPTION - IMPLICATIONS FOR THE TOXIC OIL SYNDROME, Archives of environmental contamination and toxicology, 28(4), 1995, pp. 524-528
A study was performed to examine the ability of dietary oleic anilide
to alter 12-hydroxyeicosatetraenoic acid (12-HETE) production. The str
ucture of oleic anilide, synthesized by reacting oleic acid with anili
ne, was confirmed by mass spectrometry. The purity of oleic anilide, 7
5%, was measured by gas chromatography. Oleic acid, which constituted
the remaining 25%, is a major component of the rapeseed oil vehicle. B
alb/c mice were fed oleic anilide as 0.75% of their diet by weight for
three weeks. Their lungs were excised and examined for 12-HETE produc
tion in vitro. The 12-HETE levels were significantly (p < 0.01) lower
in mice fed oleic anilide than in mice fed the oleic acid control diet
. This result illustrates eicosanoid production as a target of fatty a
cid anilide toxicity. The fatty acid composition, including arachidoni
c acid, of mouse lungs from both dietary groups was not different. Thi
s confirms the availability of substrate for 12-lipoxygenase in both g
roups. Spleen weights were higher in mice fed oleic anilide than in co
ntrol mice (p < 0.005). These observations are relevant to immunoregul
ation and the autoimmune syndromes noted in patients of the Toxic Oil
Syndrome (TOS).