SWISS POPULATION-DATA AND FORENSIC EFFICIENCY VALUES ON 3 TETRAMERIC SHORT TANDEM REPEAT LOCI, HUMTHO1, TPOX, AND CSF1PO, DERIVED USING A STR MULTIPLEX SYSTEM

Allele and genotype frequencies for 3 tetrameric short tandem repeat l oci were determined in a Swiss population sample (n = 100) using the G enePrint STR Multiplex System, electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by s ilver staining. The loci are HUMTHO1, TPOX, and CSF1PO. The observed h eterozygosities are 83.0%, 60.0%, and 72.0%, respectively. The discrim ination power determined for the individual loci is 0.914, 0.780, and 0.860, respectively, and the combined discrimination power for the tri pler is 0.997. All loci meet Hardy Weinberg expectations and after Bon ferroni correction there was no evidence that the population sample de viates from expectations of independence. Moreover, independence of al leles at these STR loci with other PCR-based loci derived from the sam e Swiss population sample, previously reported, were considered. These loci were DQA1, LDLR, GYPA, HBGG, D7S8, GC and D1S80. Again, after Bo nferroni correction there was no evidence that the population sample d eviates from expectations of independence among alleles at the 10 diff erent PCR-based loci. Thus, the allelic frequency data can be used in human identity testing to estimate the frequency of a multiple PCR-bas ed DNA profile in the Swiss population.