USE OF MONOCLONAL-ANTIBODIES TO DETECT BY ENZYME-IMMUNOASSAY AUJESZKYS-DISEASE VIRUS IN CULTURE-MEDIUM

Three modifications of quantitative enzyme immunoassay (EIA) for asses sment of Aujeszky's disease virus (ADV) in culture medium are compared , in which monoclonal antibodies (MAB) to ADV GH glycoprotein were use d: nonconcurrent two-site ''sandwich'' EIA on the basis of two differe nt MABs, indirect concurrent EIA, and direct concurrent EIA. MABs fit best of all for use in every of EIA modification were selected. Condit ions of the assays were optimized. Concurrent EIAs were 10-20 times le ss sensitive than sandwich EIA. The development sandwich EIA permitted ADV assay in preparations of both live and formaldehyde-inactivated v irus.