CYTOCHROME-C(6) FROM THE GREEN-ALGA MONORAPHIDIUM-BRAUNII - CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES

Authors
FRAZAO C DIAS JM MATIAS PM ROMAO MJ CARRONDO MA HERVAS M NAVARRO JA DELAROSA M SHELDRICK GM
Citation
C. Frazao et al., CYTOCHROME-C(6) FROM THE GREEN-ALGA MONORAPHIDIUM-BRAUNII - CRYSTALLIZATION AND PRELIMINARY DIFFRACTION STUDIES, Acta crystallographica. Section D, Biological crystallography, 51, 1995, pp. 232-234
Citations number
14
Categorie Soggetti
Crystallography,Biology,"Pharmacology & Pharmacy
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
51
Year of publication
1995
Part
2
Pages
232 - 234
Database
ISI
SICI code
0907-4449(1995)51:<232:CFTGM->2.0.ZU;2-6
Abstract
Cytochrome c(6), a plastocyanin functionally interchangeable electron carrier between the chlorophyll molecule P700 of photosystem I and cyt ochrome f from cytochrome b(6)f complex, has been isolated from the gr een alga Monoraphidium braunii and crystallized by the vapour-diffusio n technique in sodium citrate. Crystals belong to space group R3, with cell dimensions a = b = 51.93 (5) and c = 80.5 (1) Angstrom (hexagona l axes), with one molecule per asymmetric unit. They diffract beyond 1 .9 Angstrom under a Cu K alpha rotating-anode source, with an anomalou s signal that allows the positioning of the heme Fe atom in the unit c ell.