FLOW CYTOMETRIC ANALYSIS FOR CYTOKINE PRODUCTION IDENTIFIES T-HELPER-1, T-HELPER-2, AND T-HELPER-0 CELLS WITHIN THE HUMAN CD4(-) LYMPHOCYTESUBPOPULATION()CD27()

Using three-color flow cytometric analysis for the detection of intrac ellular cytokines, we have been able to determine the exact combinatio n of cytokines produced by individual T lymphocytes. Because CD4(+)CD2 7(-) lymphocytes have been shown to produce more IL-4 and IL-5 than CD 4(+)CD27(+) lymphocytes, cells from normal individuals (n = 4) and hel minth-infected patients (n = 4) were sorted magnetically for the CD4()CD27(+) and the CD4(+)CD27(-) subpopulations. Intracellular staining for IL-4, IL-5, and IFN-gamma subsequent to mitogen stimulation for 6 h revealed that although almost no CD4(+)CD27(-) lymphocytes produce b oth IL-5 and IFN-gamma (0.03-1.4%), a distinct proportion produce both IL-4 and IFN-gamma (0.1-8.0%), and 66% to 84% of IL-5-producing cells also produce IL-4. Patients and normal individuals had the same funct ional T cell subsets, but the CD4(+)CD27(-) lymphocytes from patients had higher frequencies of cells producing IL-4 (geometric mean (GM), 2 4.3% vs 16.4%) or IL-5 (CM, 10.2% vs 2.9%), whereas those of normal in dividuals had higher frequencies of cells producing IFN-gamma (GM, 44. 5% vs 17.2%; p = 0.043). These analyses also revealed that the CD4(+)C D27(-) population included significantly higher frequencies of cells t hat were IL-5(+)IFN-gamma(-) (CM, 4.9% vs 1.5%; p = 0.025), IL-4(+)IFN -gamma(-) (GM, 13.8% vs 3.5%; p = 0.025), and IFN-gamma(+)IL-4(-)IL-5( -) (GM, 27.3% vs 12.0%; p = 0.011)than the CD4(+)CD27(+) population. T hus, we have clearly demonstrated Th1, Th2, and Th0 cell subsets withi n the CD4(+)CD27(-) population of human lymphocytes.