Rg. Courteneyharris et al., COMPARISON OF THE EARLY PRODUCTION OF EXTRACELLULAR-MATRIX ON DENSE HYDROXYAPATITE AND HYDROXYAPATITE-COATED TITANIUM IN CELL AND ORGAN-CULTURE, Biomaterials, 16(6), 1995, pp. 489-495
In order to investigate the earliest changes at the bone-hydroxyapatit
e (HA) interface, two materials, dense HA and HA-coated titanium alloy
, were investigated for their ability to stimulate production of extra
cellular matrix both in cell and organ culture. Osteoblast-like cells
were obtained from rabbit calvariae after preliminary culture for 3 d
and seeded out onto the two materials. When these cells were grown in
culture on the dense HA no matrix production was seen with the scannin
g electron microscope after 4 d, but there did appear to be matrix pro
duced on the rougher surface of the HA coating. In contrast, extracell
ular matrix production was greatly increased in organ culture where th
e material was placed on a section of bone, particularly on the HA coa
ting, where it formed an organized layer of collagen within 4 d. These
results showed that extracellular matrix production by both osteoblas
tic cells and in calvarial culture was greater on the HA coatings than
dense HA. In addition, organ culture allows the investigation of the
earliest changes in the bone-HA interface without having to undertake
in vivo implantation studies of such short duration.