Two studies were conducted to investigate affective modulation of star
tle responses to unilateral tactile probes and to determine whether su
ch modulation is lateralized. Right-handed undergraduates received air
puffs to the left or right temple while viewing pleasant, neutral, and
unpleasant pictures. Side of probe presentation was varied between th
e two trial blocks of the experiment in Study 1 (n = 48) but varied ra
ndomly within trial blocks in Study 2 (n = 48). Primary results were c
onsistent across studies. Replicating and extending the findings for a
coustic probes, eyeblink responses to tactile probes were larger durin
g unpleasant than during pleasant pictures. However, affective modulat
ion of startle did not differ reliably between the two sides of probe
presentation (sensory laterality) or the two sides of the response (mo
tor laterality) in either study or in a combined analysis.