RELATIONSHIP BETWEEN RETENTION VALUES AND SOLVATOCHROMIC PARAMETERS IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

Quantitative relationship between the retention values, the parameters a and c in retention equation lnk'=a+cC(b) and the solvatochromatic p arameters in reversed-phase liquid chromatography (RP-HPLC) have been studied. The capacity factors of polar and non-polar solutes at differ ent organic modifier concentration can be predicted by the solvatochro matic parameters through the prediction of parameters a and c. From th e quantitative relationships of parameters a and c between the acetoni trile/water and methanol/water mobile phases, it is shown that the hyd rogen bonding solvatochromatic parameters play a significant role in i mprovement of the quantitative relationship.