ELECTROCHEMICAL-BEHAVIOR, APPLICATION AND IMMOBILIZATION OF GLUCOSE-OXIDASE AT NANOMETER-SIZED ULTRAMICROBAND GOLD ELECTRODE

The immobilization of glucose oxidase, as well as simultaneous immobil ization of glucose oxidase and Fe(CN)(6)(3-) as an electron mediator i n polypyrrole film was successfully accomplished at a nanometer-sized ultramicroband gold electrode to provide a simple biosensor for detect ing glucose without mediator in solution. The GOD/PPy electrode was ch aracterized by IR spectrum and cyclic voltammetry. The electropolymeri zation process of pyrrole and the effects of glucose oxidase on the el ectropolymerization pyrrole were also studied with chronoamperometry. The effects of polypyrrole thickness and oxygen in solution on the det ermination of glucose at GOD/Fe(CN)(6)(3-)/PPy microelectrode were dis cussed.