THEORETICAL-STUDIES ON BIOSPECIFIC ADSORPTION FOR LARGE-SCALE AFFINITY SEPARATIONS

A dynamic model has been developed in order to estimate values of the parameters characterizing the interaction of transport phenomena with biospecific adsorption in Bred-bed affinity separations. The derived d imensionless model equations comprise the hydrodynamic behaviour on th e basis of the Bodenstein number, influences of mass transfer by the S tanton number and biochemical driving potentials of the adsorption by the Gamma number. Model parameters were estimated on the basis of expe rimental results obtained from fixed-bed experiments. Results obtained from fixed-bed experiments and model predictions show that transport resistances have an important influence on the efficiency of biospecif ic separations and have to be considered in the design of large-scale systems as well as in the development of functionalized supports. Copy right (C) 1996 Elsevier Science Ltd