COMPLEX-FORMATION BETWEEN GAMMA-IMMUNOGLOBULIN AND CALMODULIN IN CALCIUM-FREE CONDITIONS

Authors
TAKEUCHI Y BIRCKBICHLER PJ PATTERSON MK
Citation
Y. Takeuchi et al., COMPLEX-FORMATION BETWEEN GAMMA-IMMUNOGLOBULIN AND CALMODULIN IN CALCIUM-FREE CONDITIONS, Experientia, 51(4), 1995, pp. 339-342
Citations number
14
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Experientia → ACNP
ISSN journal
00144754
Volume
51
Issue
4
Year of publication
1995
Pages
339 - 342
Database
ISI
SICI code
0014-4754(1995)51:4<339:CBGACI>2.0.ZU;2-N
Abstract
We show that gamma-immunoglobulin (IgG) binds calmodulin (CaM) in a Ca 2+-independent manner, with Kd value of (1.7 +/- 0.5) x 10(-7) M. A si ngle IgG molecule maximally bound 10 CaM molecules. The binding is to the heavy chain or Fab portion, but not the Fc portion, of the IgG mol ecules. Ca2+ greatly diminished the interaction between IgG and CaM, w ith IC50 = 8-9 mu M. These data give a novel insight into protein-prot ein interactions.