A(2) ADENOSINE RECEPTORS IN HUMAN GLOMERULAR MESANGIAL CELLS

A(2) adenosine receptors were characterized in human glomerular mesang ial cells using [H-3] 5'-N-ethylcarboxamidoadenosine (NECA) as a trace r. There was a single group of receptor sites with a K-D of 184 nM, an d a number of sites of 317 fmol/mg of cell protein. Adenosine agonists increased 5'-nucleotidase activity via A(2) receptor stimulation. The specific A(2) agonist-NECA, at 0.1 and 1 mu m, was a potent inhibitor of DNA synthesis.