Ag. Rao et al., IDENTIFICATION AND FIELD OPTIMIZATION OF THE FEMALE SEX-PHEROMONE OF THE RICE LEAFFOLDER, CNAPHALOCROCIS-MEDINALIS IN INDIA, Entomologia experimentalis et applicata, 74(3), 1995, pp. 195-200
Analysis of ovipositor washings from virgin female Cnaphalocrocis medi
nalis Guenee (Lepidoptera: Pyralidae) of Indian origin by linked gas c
hromatography and electroantennography indicated the presence of three
electrophysiologically-active compounds. These were identified on the
basis of their gas chromatographic retention times and mass spectra a
s (Z)-11-hexadecenyl acetate, (Z)-13-octadecenyl acetate and (Z)-13-oc
tadecen-1-ol with (Z)-13-octadecenyl acetate present in amounts of bet
ween 0.25 and 1.5 ng per ovipositor and the other two components at le
ss than 10% of this. Trace quantities of octadecyl acetate were identi
fied by mass spectrometry but no electroantennographic responses were
observed to this compound. Field trials conducted with a range of blen
ds of the three electrophysiologically-active compounds showed that bl
end containing between 5% and 30% (Z)-11-hexadecenyl acetate in (Z)-13
-octadecenyl acetate dispensed from either white rubber septa or polyt
hene vials were more attractive to male moths than a virgin female mot
h. Addition of (Z)-13-octadecen-1-ol reduced attractiveness to male mo
ths in the blends and concentrations tested.