DIFFICULTIES IN OBTAINING MONOCLONAL-ANTIBODIES TO SUBSETS OF HUMAN-LEUKOCYTES, USING NEONATAL TOLERANCE INDUCTION IN MICE

Many antigens are shared between different types of human leukocytes. In an effort to obtain new lineage-specific monoclonal antibodies, par ticularly antibodies to dendritic cells, we attempted to tolerize newb orn mice to one type of leukocyte and then immunize the adults with an other. We found that T cells, either unstimulated T cells or T blasts, were more effective at inducing neonatal tolerance than non-T cells o r B cell lines. However, the tolerance that was achieved was not restr icted to T cells, since we could not elicit from the tolerized mice a specific antibody response to a B cell line or to blood dendritic cell s. Here we describe several efforts, all unsuccessful, to achieve cell specific immune responses in tolerant mice. The parameters we conside red included the type of cell used to tolerize neonatal mice, the regi men of injections for inducing tolerance or eliciting immunity, and th e use of several different adjuvants.