CLONING, NUCLEOTIDE-SEQUENCE, AND TRANSCRIPTIONAL ANALYSIS OF THE NUSG - GENE OF STREPTOMYCES-COELICOLOR A3(2), WHICH ENCODES A PUTATIVE TRANSCRIPTIONAL ANTITERMINATOR
C. Puttikhunt et al., CLONING, NUCLEOTIDE-SEQUENCE, AND TRANSCRIPTIONAL ANALYSIS OF THE NUSG - GENE OF STREPTOMYCES-COELICOLOR A3(2), WHICH ENCODES A PUTATIVE TRANSCRIPTIONAL ANTITERMINATOR, MGG. Molecular & general genetics, 247(1), 1995, pp. 118-122
A 3 kb genomic fragment containing the nusG gene of Streptomyces coeli
color A3(2) was identified, cloned and sequenced. Sequence analysis re
vealed 3 complete and 2 truncated open reading frames (ORFs): truncate
d ORF(U) (similar to a Bacillus gene encoding a thermostable aspartate
aminotransferase)-secE (94 amino acids; 79.0% similarity to Escherich
ia coli SecE)-nusG (300 amino acids; 73.3% similarity to E. coli NusG)
-rplK (144 amino acids; 88.5% similarity to E. coli ribosomal subunit
L11)-truncated rplA (similar to E. coli ribosomal subunit L1). The gen
e organization secE-nusG-rplKA exactly matches that in E. coli. Transc
riptional analyses by the primer extension method revealed one transcr
iptional start site each for secE and nusG, and two sites for rplK. Th
e presence of promoters was also confirmed with the aid of a promoter-
probe vector.