SITE-SPECIFIC N-15-LABELING OF OLIGONUCLEOTIDES FOR NMR - THE TRP OPERATOR AND ITS INTERACTION WITH THE TRP REPRESSOR

Authors
RAMESH V XU YZ ROBERTS GCK
Citation
V. Ramesh et al., SITE-SPECIFIC N-15-LABELING OF OLIGONUCLEOTIDES FOR NMR - THE TRP OPERATOR AND ITS INTERACTION WITH THE TRP REPRESSOR, FEBS letters, 363(1-2), 1995, pp. 61-64
Citations number
25
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
363
Issue
1-2
Year of publication
1995
Pages
61 - 64
Database
ISI
SICI code
0014-5793(1995)363:1-2<61:SNOOFN>2.0.ZU;2-N
Abstract
A convenient and economical method is described for the site-specific N-15-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide c orresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using H-1-N-15 HMQ C and N-15-edited H-1-H-1 NOESY experiments.