EXPRESSION AND FUNCTIONAL-PROPERTIES OF THE 2ND PREDICTED NUCLEOTIDE-BINDING FOLD OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR FUSED TO GLUTATHIONE-S-TRANSFERASE
C. Randak et al., EXPRESSION AND FUNCTIONAL-PROPERTIES OF THE 2ND PREDICTED NUCLEOTIDE-BINDING FOLD OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR FUSED TO GLUTATHIONE-S-TRANSFERASE, FEBS letters, 363(1-2), 1995, pp. 189-194
CFTR-NBF-2 was expressed in Escherichia coli in fusion with glutathion
e-S-transferase, the soluble portion was purified and identified as a
stuctured protein by its CD spectrum. Association reactions of the rec
ombinant NBF-2 with adenine nucleotides were monitored qualitatively b
y demonstrating its ability to bind specifically to ATP-, ADP- and AMP
-affinity agarose and quantitatively by recording the fluorescence enh
ancement of excited trinitrophenol (TNP)-labelled adenine nucleotides
occuring as a result of binding to NBF-2. Best-fit monophasic binding
curves to the fluorescence data indicated K-d values of 22 mu M for TN
P-ATP, 39 mu M for TNP- ADP and 2.1 mu M for TNP-AMP. The corrected K-
d values for unlabelled adenine nucleotides competing with the fluorop
hores mere determined to be 37 mu M for ATP, 92 mu M for ADP and 12 mu
M for AMP. The recombinant NBF-2 did not show any hydrolytic activity
on ATP (detection limit 0.001 s(-1)). Our findings support the concep
t of a central role of NBF-2 in CFTR activity regulation acting as an
allosteric switch between channel opening and dosing and give the firs
t experimental evidence that the channel inhibitor AMP could act via N
BF-2.