ALTERED REPLICATION AND INVERTED REPEATS INDUCE MISMATCH REPAIR-INDEPENDENT RECOMBINATION BETWEEN HIGHLY DIVERGED DNAS IN YEAST

Citation
H. Tran et al., ALTERED REPLICATION AND INVERTED REPEATS INDUCE MISMATCH REPAIR-INDEPENDENT RECOMBINATION BETWEEN HIGHLY DIVERGED DNAS IN YEAST, Molecular and cellular biology, 17(2), 1997, pp. 1027-1036
Citations number
59
Categorie Soggetti
Biology,"Cell Biology
ISSN journal
02707306
Volume
17
Issue
2
Year of publication
1997
Pages
1027 - 1036
Database
ISI
SICI code
0270-7306(1997)17:2<1027:ARAIRI>2.0.ZU;2-D
Abstract
Replication, DNA organization, and mismatch repair (MMR) can influence recombination. We examined the effects of altered replication due to a mutation in the polymerase delta gene, long inverted repeats (LIRs) in motifs similar to those in higher eukaryotes, and MMR on intrachrom osomal recombination between highly diverged (28%) truncated genes in Saccharomyces cerevisiae. A combination of altered replication and an LIR increased recombination up to 700-fold, while each alone led to a 3- to 20-fold increase. Homeologous recombination was not altered by p ms1, msh2, and msh3 mismatch repair mutations. Similar to our previous observations for replication slippage-mediated deletions, there were greater than or equal to 5-bp identical runs at the recombination brea kpoints. We propose that the dramatic increase in recombination result s from enhancement of the effects of altered replication by the LIR, l eading to recombinationally active initiating structures. Such interac tions predict replication-related, MMR-independent genome changes.