IDENTIFICATION AND MINISATELLITE LINKAGE ANALYSIS OF SMXA RECOMBINANTINBRED STRAINS OF MICE BY DNA-FINGERPRINTING

SMXA recombinant inbred (RI) strains of mice were produced by systemat ic inbreeding from the F2 generation of a cross between two progenitor inbred strains, A/J and SM/J, which differ considerably with respect to many characteristics, and consists of 28 inbred strains. In this st udy, we investigated the applicability of DNA fingerprinting with M13 phage DNA to the identification of these closely related strains. DNA fingerprints of the SMXA RI strains and their progenitors, SM/J and A/ J, showed strain-specific patterns, with the same banding patterns wit hin each strain. Linkage analysis by using strain distribution pattern s of minisatellite loci with 108 genetic markers containing microsatel lites, biochemical and immunological marker genes allowed 23 minisatel lite loci to be assigned to 11 chromosomes. The results suggested that DNA fingerprinting with M13 phage DNA is applicable not only for stra in identification but also for genetic monitoring of RI strains on alm ost all chromosomes.