CLONING AND FUNCTIONAL-CHARACTERIZATION OF THE HUMAN VASOACTIVE-INTESTINAL-PEPTIDE (VIP)-2 RECEPTOR

A full-length clone encoding the human VIP-2 receptor was isolated fro m a human placenta cDNA Library. The 1317-bp cDNA insert encodes a pro tein of 438 amino acids which is a member of the seven transmembrane d omain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [I-125 ] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC5 0 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulat ion of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skelet al muscle and to a lesser extent in the human brain, heart, pancreas a nd placenta. (C) 1995. Academic Press, Inc.