Staphylococcus (S.) simulans, S. chromogenes, and S. epidermidis were
the species of coagulase-negative staphylococci (CNS) most frequently
involved in subclinical mastitits of dairy cattle. The same biochemica
l features characterized the successive isolates of a particular chron
ically infected udder quarter. Occurrence rate of the various species
differed between the herds examined. In cases of clinical mastitis of
cows S. xylosus, S. simulans, S. haemolyticus, and S. chromogenes pred
ominated. A comparison between strains of the particular CNS species f
rom infections in man and cattle did not show host attributable bioche
mical features or profiles obtained by polymerase chain reaction using
arbitrary primers (AP-PCR).