PURIFICATION AND PROPERTIES OF A CYTOLYTIC TOXIN IN VENOM OF THE JELLYFISH CARYBDEA-MARSUPIALIS

Authors
ROTTINI G GUSMANI L PAROVEL E AVIAN M PATRIARCA P
Citation
G. Rottini et al., PURIFICATION AND PROPERTIES OF A CYTOLYTIC TOXIN IN VENOM OF THE JELLYFISH CARYBDEA-MARSUPIALIS, Toxicon, 33(3), 1995, pp. 315-326
Citations number
22
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
33
Issue
3
Year of publication
1995
Pages
315 - 326
Database
ISI
SICI code
0041-0101(1995)33:3<315:PAPOAC>2.0.ZU;2-2
Abstract
A haemolytic toxin was purified by ion-exchange chromatography and FPL C gel filtration from the nematocysts of the jellyfish Carybdea marsup ialis. Sheep red cells, but not human or rabbit red cells, were suscep tible to lysis by the toxin. The toxin is a protein with an apparent m olecular mass of about 102-107 kDa, is heat labile, highly unstable in polar media, inactivated by reducing agents, and devoid of phospholip ase activity. The experimental data speak in favour of a pore-forming mechanism of toxin action.