Two isolates of encephalomyocarditis (EMC) virus (ZRC 276RA/90 and ZRC
292RA/90) were isolated from two dormice (Myoxus glis) in Tuscany, It
aly. The two isolates were lethal for laboratory mice and caused a rap
id cytopathic effect characterized by rounded and wrinkled cells in bo
th baby hamster kidney cells (BHK21) and African green monkey kidney c
ells (Vero). We found neutralizing antibodies against EMC virus in 408
(77%) of 529 domestic pigs (Sus scrofa scrofa) and in 165 (49%) of 33
8 wild boars (S. scrofa ferus majori) in Tuscany.