CLONING AND SEQUENCING OF THE CDNA-ENCODING THE PITUITARY GONADOTROPIN-II BETA-SUBUNIT OF YELLOWFIN PORGY (ACANTHOPAGRUS-LATUS)

The complementary DNA (cDNA) encoding the gonadotropic hormone (GTH) I I pre-beta-subunit of yellowfin porgy (Acanthopagrus latus) was isolat ed from a pituitary gland cDNA phage library. The cDNA insert, 598 bas e pairs (bp), contained a 411 bp open reading frame with 35 bp and 152 bp flanking regions at the 5'- and 3'-ends, respectively. The deduced amino acid sequence revealed a putative signal peptide of 24 amino ac id residues and a 113 amino acid mature beta-subunit of GTH polypeptid e. This pre-beta-subunit polypeptide of the yellowfin porgy GTH II sho wed 86% sequence identity with that of bonito GTH II-beta, 73% with ki llifish GTH II-beta, 63% with African catfish GTH II-beta, 61% with pi ke eel GTH-beta, 60% with silver carp GTH-beta, 59% with chum salmon G TH II-beta, 58% with common carp GTH-beta, 57% with chinook Pacific sa lmon GTG-beta, and 53% with European eel GTH II-beta.