THE PERMEABILITY OF FRESH PREOVULATORY HUMAN OOCYTES TO DIMETHYL-SULFOXIDE AT 3-DEGREES-C

Authors
MCGRATH JJ FULLER BJ HUNTER JE PAYNTER S BERNARD AG
Citation
Jj. Mcgrath et al., THE PERMEABILITY OF FRESH PREOVULATORY HUMAN OOCYTES TO DIMETHYL-SULFOXIDE AT 3-DEGREES-C, Cryo-letters, 16(2), 1995, pp. 79-84
Citations number
18
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
16
Issue
2
Year of publication
1995
Pages
79 - 84
Database
ISI
SICI code
0143-2044(1995)16:2<79:TPOFPH>2.0.ZU;2-E
Abstract
The Kedem-Katchalsky (K-K) parameters of hydraulic conductivity (L(p)) , cryoprotectant permeability (omega) and reflection coefficient (sigm a) have been determined for five fresh preovulatory human oocytes expo sed to 1.5 M dimethyl sulfoxide (DMSO) at 3 degrees C. The mean values of these parameters are: L(p) = 0.43 mu m/min atm; omega = 0.16 mu m/ sec; sigma = 0.82. These permeability values are the first reported va lues for human oocytes at low temperatures. The results are discussed in relation to previous studies of human and murine oocytes.