W. Wang et al., EFFECT OF THE CONCENTRATION OF DODMAC AND 1-DECANOL ON THE BEHAVIOR OF REVERSE MICELLES IN THE EXTRACTION OF AMINO-ACIDS, Biotechnology and bioengineering, 46(4), 1995, pp. 343-350
The concentrations of dioctyldimethyl ammonium chloride (DODMAC) and 1
-decanol in isooctane needed to form reverse micelles by phase contact
have been determined. The behavior of these reverse micelles in the e
xtraction of aspartic acid, glutamic acid, and threonine was studied b
y analyzing all of the ionic species in the aqueous phase. The amino a
cid is extracted from the aqueous phase by exchanging with the Cl- cou
nterions of DODMAC in the reverse micelles. The ionic species in the r
everse micelles tend toward their undissociated states as the water up
take by the reverse micelles decreases. The effect of 1-decanol on the
extraction of the amino acids with two negative charges is due to the
change in the water uptake of the reverse micelles. The concentration
of DODMAC has no effect on the ion exchange of the amino acid with on
e negative charge with the Cl- counterions of DODMAC in the reverse mi
celles. Higher molar ratios of decanol to DODMAC favor the selective s
eparation of amino acids with different charge numbers. (C) 1995 John
Wiley and Sons, Inc.