L. Torchio et al., DETERMINATION OF THE POLAR DRUG DIMIRACETAM IN HUMAN PLASMA AND SERUMBY COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 666(1), 1995, pp. 169-177
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
A sensitive and fully automated column-switching HPLC method was devel
oped for the determination of a novel cognition enhancer, dimiracetam,
in human plasma and serum. A sample of plasma was ultrafiltered and t
hen directly injected onto a first mu Bondapak C-18 column. The sample
was eluted with water. The portion of the eluate (heart-cut) from thi
s column containing the drug was selected and loaded on a second mu Bo
ndapak C-18 column and eluted with water. During the elution of the se
cond column, the first column was automatically rinsed with water-acet
onitrile (50:50, v/v) and then equilibrated for a new injection. The t
otal analysis time for each sample was 35 min. This corresponded to a
30% decrease in analysis time compared with the time that would have b
een needed with a simple run with two mu Bondapak C-18 columns assembl
ed in series. Ultraviolet detection at 200 nm was used to monitor the
eluate. The method was fully validated over the quantitation range 27
ng/ml-15 mu g/ml. The applicability of the method was demonstrated by
analysis of serum samples from a study performed in human healthy volu
nteers.