COMPARISON OF THE ACTION OF 17-BETA-ESTRADIOL AND PROGESTINS WITH INSULIN-LIKE GROWTH FACTORS-I -II AND TRANSFORMING GROWTH-FACTOR-BETA-1 ON THE GROWTH OF NORMAL ADULT HUMAN BONE-FORMING CELLS/
Hjj. Verhaar et al., COMPARISON OF THE ACTION OF 17-BETA-ESTRADIOL AND PROGESTINS WITH INSULIN-LIKE GROWTH FACTORS-I -II AND TRANSFORMING GROWTH-FACTOR-BETA-1 ON THE GROWTH OF NORMAL ADULT HUMAN BONE-FORMING CELLS/, Maturitas, 21(3), 1995, pp. 237-243
Citations number
31
Categorie Soggetti
Geiatric & Gerontology","Obsetric & Gynecology","Medicine, General & Internal
Endogenous growth factors may be involved in the prevention of bone lo
ss by estrogen and progestins in postmenopausal women. The present stu
dy was performed to compare the action of estrogen/progestins on bone-
derived cells with the effects of exogenously added purified growth fa
ctors. Human osteoblast-like (HOB) cells were incubated with 17 beta-e
stradiol (E(2)), progesterone (P), dydrogesterone (DD), 20 alpha-dihyd
roxydydrogesterone (DHD), with and without the growth factors, insulin
-like growth factors-I/-II(IGF-U-II) or transforming growth factor-bet
a type 1 (TGF-beta 1) for 24 h under serum-free conditions. Cell growt
h and DNA synthesis were assessed by spectophotometrical analysis of t
otal cell number and immunochemical detection of BrdU incorporation, r
espectively. Compared with the sex steroids, incubation of the cells w
ith ICE-I or TGF-beta 1 resulted in at least a two-fold increase of to
tal HOB cell numbers. No difference in stimulating HOB growth was obse
rved between IGF-II and the female sex steroids E(2) and P. Combining
IGF-I/-II or TGF-beta 1 with either E(2) or P did not result in a sign
ificantly further increase in the human osteoblast-like cell growth. I
n conclusion, the bone anabolic growth factors, IGF-I and TGF-beta 1,
may be more important regulators of osteoblast proliferation than the
female sex steroids. An interaction of estrogen/gestagens with the gro
wth factors IGF-I/-II or TGF-beta 1 was not evident from the growth of
human bone-forming cells in short-term cultures.