Jr. Rayburn et al., CHARACTERIZATION OF GRASS SHRIMP (PALAEMONETES PUGIO) EMBRYO TOXICITYTESTS USING THE WATER-SOLUBLE FRACTION OF NUMBER 2 FUEL-OIL, Marine pollution bulletin, 32(12), 1996, pp. 860-866
Toxicity test procedures using grass shrimp Palaemonetes pugio exposed
to water soluble fraction (WSF) of Number 2 fuel oil were modified to
improve test utility and efficiency. The original test procedure, a 1
2-day embryo exposure period in glass tubes, was compared with modifie
d 4-day and 12-day tests in plastic tissue culture plates. Comparison
of LC(50) values, coefficients of variation and time to hatch indicate
d no significant change in test sensitivity due to the plastic contain
ers, Sensitivity was slightly reduced by shortening the test to a 4-da
y exposure period initiated 9 days after oviposition, This exposure pe
riod encompassed sensitive life stages of the developing organism. Usi
ng the 4-day exposure in plastic plates, variability due to different
broods and preparations of WSF was characterized.