ACETALDEHYDE-SERUM PROTEIN ADDUCTS INHIBIT INTERLEUKIN-2 SECRETION INCONCANAVALIN A-STIMULATED MURINE SPLENOCYTES - A POTENTIAL COMMON PATHWAY FOR ETHANOL-INDUCED IMMUNOMODULATION
Kp. Braun et al., ACETALDEHYDE-SERUM PROTEIN ADDUCTS INHIBIT INTERLEUKIN-2 SECRETION INCONCANAVALIN A-STIMULATED MURINE SPLENOCYTES - A POTENTIAL COMMON PATHWAY FOR ETHANOL-INDUCED IMMUNOMODULATION, Alcoholism, clinical and experimental research, 19(2), 1995, pp. 345-349
Variable immunobiological changes occur with alcohol consumption. Prev
ious studies have shown that acetaldehyde forms stable adducts with se
rum proteins, including albumin. These adducts are elevated in persons
and animals consuming ethanol. We examined the effect of serum protei
n-acetaldehyde adducts formed with fetal bovine serum (FBS) on concana
valin A-stimulated murine splenocytes. Interleukin-2 (IL-2) secretion
and IL-2 receptor (IL-2R) expression were determined as a function of
the effect of the acetaldehyde-protein adduct(s), FBS was incubated wi
th acetaldehyde (500, 100, 50, 25, 10, and 0 mu M) for 1 hr at 37 degr
ees C. Excess acetaldehyde was removed by ultrafiltration using a 500
molecular weight cut-off membrane in 3 volumes. Free as well as bound
acetaldehyde was quantified using fluorigenic HPLC before and after in
cubation. Recovered acetaldehyde correlated with the amount added (r(2
) = 0.996). Splenocytes were cultured for 48 hr in complete medium con
taining 5% acetaldehyde-treated and 5% untreated FBS with 4 mu g/ml co
ncanavalin A. Although cell viability was unchanged, acetaldehyde-trea
ted FBS mixed with native FBS decreased IL-2 secretion in a dose-depen
dent manner. The percentage of cells expressing IL-2R was reduced only
at the highest acetaldehyde-fas dose. Therefore, immunological effect
s ascribed to ethanol may result in part from the toxic properties of
acetaldehyde-protein adducts on IL-2 secretion.