ELECTROPHORETIC ANALYSIS OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) LEUKOCYTE LYSATES

Thymus, spleen, kidney and peripheral blood leucocyte lysates subjecte d to SDS-PAGE showed obvious differences in the protein patterns. Thym ic lysate patterns showed the presence of a 38 kDa protein which was n ot present in any of the other samples. It was found that this protein was susceptible to hydrocortisone treatment. Adult thymectomy was fou nd to alter the SDS-PAGE protein patterns from spleen, kidney and peri pheral blood leucocyte lysates. In intact fish a 16.5 kDa and a 13.5 k Da protein were present in thymus, spleen, peripheral blood and kidney lysates, but was absent from spleen, peripheral blood and kidney lysa tes of fish thymectomised 6 months previously.