Ng. Kolosova et al., THE CHANGES OF CONTENT, STRUCTURE AND CHA RGE OF RAT PLASMA-LIPOPROTEINS DURING LONG-TERM COLD-EXPOSURE, Biofizika, 40(2), 1995, pp. 422-427
The paper shows the results of complex investigation with the fluoresc
ence techniques, electrophoresis. a.o. methods of rat plasma lipoprote
ins in various terms (1 day - 49 days) of cold exposure (+5 degrees C)
. There was the quantitative increase of the common fractions of VLDL
and LDL to the 15 days of cold acclimation. The variations in percenta
ge composition of LP occured already on the first day. The changes of
physico-chemical features of LP (surface charge and surface area, lipi
d viscosity and protein-lipid interactions did not always correspond t
o their quantitative changes and were not connected with lipid peroxid
ation. The decrease of the charges and the diminution of the surface a
rea of VLDL, LDL and HDL were revealed on the first day. At 28-49 days
the charges of VLDL and LDL were higher than in control, but LDL' one
was lower. The occured at this term decrease of efficiency of fluores
cence energy transfer from LP' proteins to lipids, different variation
s of lipid viscosity demonstrated the less dip of proteins into lipids
and/or the forming the aggregates of protein molecules. These in vivo
LP modifications undoubtedly can induce the changes of their receptiv
e and regulatory abilities.