Ar. Holmes et al., ADHERENCE OF CANDIDA-ALBICANS TO A CELL-SURFACE POLYSACCHARIDE RECEPTOR ON STREPTOCOCCUS-GORDONII, Infection and immunity, 63(5), 1995, pp. 1827-1834
Candida albicans ATCC 10261 and CA2 bound to cells of the oral bacteri
a Streptococcus gordonii, Streptococcus oralis, and Streptococcus sang
uis when these bacteria were immobilized onto microtiter plate wells,
but they did not bind to cells of Streptococcus mutans or Streptococcu
s salivarius, Cell wall polysaccharide was extracted with alkali from
S. gordonii NCTC 7869, the streptococcal species to which C, albicans
bound with highest affinity, and was effective in blocking the coaggre
gation of C. albicans and S. gordonii cells in the fluid phase. When f
ixed to microtiter plate wells, the S, gordonii polysaccharide was bou
nd by all strains of C. albicans tested, The polysaccharide contained
Rha, Glc, GalNAc, GlcNAc, and Gal and was related compositionally to p
reviously characterized cell wall polysaccharides from strains of S. o
ralis and S. sanguis. The adherence of yeast cells to the immobilized
polysaccharide was not inhibitable by a number of saccharides, Antiser
um raised to the S. gordonii NCTC 7869 polysaccharide blocked adherenc
e of C. albicans ATCC 10261 to the polysaccharide, The results identif
y a complex cell wall polysaccharide of S. gordonii as the coaggregati
on receptor for C. albicans. Adherent interactions of yeast cells with
streptococci and other bacteria may be important for colonization of
both hard and soft oral surfaces by C. albicans.