The atomic force microscope has become a standard tool to measure surf
ace forces, Force-versus-distance curves taken with an atomic force mi
croscope often show a hysteresis in the noncontact region between appr
oach and retraction. The hysteresis can be caused by the discrete, ste
pwise motion of the sample due to digitalization, Since in the presenc
e of liquids cantilever and sample are coupled, the cantilever oscilla
tes after each step. Depending on when a data point is recorded during
this oscillation, the amplitude of the cantilever deflection measured
may deviate from the equilibrium value. This might cause a hysteresis
and other misleading results in force-versus-distance measurements. E
specially when attractive forces are measured, cantilever oscillations
can severely change the results.