EVALUATION OF DRUG LIPID ASSOCIATION CONSTANTS FROM MICROELECTROPHORETIC MOBILITY MEASUREMENTS

Authors
BANERJEE S CASPERS J BENNOUNA M SAUTEREAU AM TOCANNE JF RUYSSCHAERT JM
Citation
S. Banerjee et al., EVALUATION OF DRUG LIPID ASSOCIATION CONSTANTS FROM MICROELECTROPHORETIC MOBILITY MEASUREMENTS, Langmuir, 11(4), 1995, pp. 1134-1137
Citations number
42
Categorie Soggetti
Chemistry Physical
Journal title
Langmuir → ACNP
ISSN journal
07437463
Volume
11
Issue
4
Year of publication
1995
Pages
1134 - 1137
Database
ISI
SICI code
0743-7463(1995)11:4<1134:EODLAC>2.0.ZU;2-I
Abstract
Electrophoretic mobilities of negatively charged lipid vesicles were m easured after incubation with cationic drugs (adriamycin, celiptium, e thidium bromide, tetracaine) at different drug concentrations. We show here that comparison of the measured electrophoretic mobility with ad sorption isotherms allows calculation of the drug-binding constants ov er a large range of drug concentrations, whatever the structure of the drug, its topology into the lipid bilayer, or its aggregation state.