DESORPTION OF IONS FROM LOCUST TISSUES .3. STUDY OF A METABOLITE OF A-DESTRUXIN USING FAST-ATOM-BOMBARDMENT LINKED-SCAN MASS-SPECTROMETRY

The process of the detoxification of A-destruxin into a polar linear p eptide in vivo in Locusta migratoria has been demonstrated using both high performance liquid chromatography and negative-ion fast-atom bomb ardment mass spectrometry. The final structure of the metabolite, afte r synthesis and comparison of retention time with the component in the crude biological mixture, was elucidated by positive-ion fast-atom bo mbardment linked-scan mass spectrometry. Diagnostic fragment ions prod uced by collision-induced dissociation are discussed.