Rc. Bergan et al., INHIBITION OF PROTEIN-TYROSINE KINASE-ACTIVITY IN INTACT-CELLS BY THEAPTAMERIC ACTION OF OLIGODEOXYNUCLEOTIDES, Antisense research and development, 5(1), 1995, pp. 33-38
Citations number
18
Categorie Soggetti
Medicine, Research & Experimental","Biothechnology & Applied Migrobiology
Direct interaction of oligodeoxynucleotides (ODNs) with proteins repre
sents one of the nonantisense-mediated effects of ODNs. Phosphorothioa
te-capped ODNs have been shown to inhibit directly the in vitro kinase
activity of the chronic myelogenous leukemia-associated protein-tyros
ine kinase p210(bcr-abl). In this study we have determined the efficac
y of this aptameric ODN in a cellular system using the K562 chronic my
elogenous leukemia-derived cell line, Significant effects upon cellula
r phosphotyrosine content, as well as cellular growth in soft agar, ar
e observed. These effects are sequence specific and are not mediated t
hrough changes in p2l0(bcr-abl) protein levels. Additional ODNs are de
scribed that also reduce cellular phosphotyrosine levels and inhibit g
rowth in soft agar but do not inhibit p210(bcr-abl) kinase activity in
vitro.