Vitamin B-12 Concentrations were measured in plasma and liver, and cob
alt (Go) concentrations determined in faeces, of sheep given intrarumi
nal Co pellets with or without steel grub screws (grinders). The Co pe
llets contained 30 or 60% by weight Co oxide (Co3O4) Sheep were mainta
ined on pasture at 2 sites in the South East of South Australia in an
area where sheep have been shown to respond to Co supplementation. All
pellets, apart from one given without a grinder, maintained above-ade
quate vitamin B-12 concentrations in liver (>200 nmol/kg wet weight) a
nd plasma (>400 pmol/L) for 1 year. In the second year, some pellets c
ontaining 30% Co3O4 failed to maintain adequate vitamin B-12 concentra
tions in the liver of sheep at 1 site when untreated sheep were at ris
k of seasonal Co deficiency (liver vitamin B-12 Concentrations <100 nm
ol/kg wet weight). Under these circumstances, a more recently formulat
ed pellet containing 30% Co3O4, when given with a grinder, maintained
adequate vitamin Bit status of sheep for 3 years. The 'traditional' pe
llet containing 60% Co3O4 increased vitamin B-12 Concentrations to hig
h normal values (in plasma >1500 pmol/L, in liver >200 nmol/kg wet wei
ght) for at least 3 years. Pellets containing 30% Co3O4 may only preve
nt Co deficiency for 1 year, depending on the source of the pellets.