Ajg. Mank et al., DETERMINATION OF THE ANTICANCER DRUG METABOLITE WR1065 USING PRECOLUMN DERIVATIZATION AND DIODE-LASER INDUCED FLUORESCENCE DETECTION, Journal of pharmaceutical and biomedical analysis, 13(3), 1995, pp. 255-263
A liquid chromatographic (LC) procedure using alumina as stationary ph
ase in both the pre- and the analytical column, is reported for the de
termination of WR1065, the active metabolite of the amino- and thiol-c
ontaining anticancer drug WR2721. After pre-column derivatization of t
he thiol group, the analyte is determined by LC with diode laser induc
ed fluorescence detection in the near-infrared. Selective removal of e
xcess label is achieved by means of column switching; it allows the de
tection of 5 x 10(-9) M WR1065 in water and 10-fold diluted, deprotein
ated plasma samples. The detection limit is determined by the derivati
zation reaction and not by the fluorescence detection of the labelled
analyte. Endogeneous thiols do not interfere.