M. Wakamiya et al., DISRUPTION OF THE ADENOSINE-DEAMINASE GENE CAUSES HEPATOCELLULAR IMPAIRMENT AND PERINATAL LETHALITY IN MICE, Proceedings of the National Academy of Sciences of the United Statesof America, 92(9), 1995, pp. 3673-3677
We have generated mice with a null mutation at the Ada locus, which en
codes the purine catabolic enzyme adenosine deaminase (ADA, EC 3.5.4.4
), ADA-deficient fetuses exhibited hepatocellular impairment and died
perinatally, Their lymphoid tissues were not largely affected, Accumul
ation of ADA substrates was detectable in ADA-deficient conceptuses as
early as 12.5 days postcoitum, dramatically increasing during late in
utero development, and is the likely cause of liver damage and fetal
death. The results presented here demonstrate that ADA is important fo
r the homeostatic maintenance of purines in mice.